Carbon and nitrogen stable isotope ratios and elemental composition of specimens from the pelagic food web collected during the 2019 International Year of the Salmon expedition to the Gulf of Alaska

All samples were collected on board of the chartered R/V Professor Kaganovsky between February and March 2019 during the winter expedition of the International Year of the Salmon project to the Gulf of Alaska, North Pacific Ocean. Samples of Particulate Organic Matter (POM) were obtained by filtering approximately 2 L of water onto pre-combusted 25 mm GF/F filters with a vacuum pump. Water samples were collected at every station from 5m depth using Niskin bottles. Two replicates were filtered per station, one subsample was acidified by immersion in 1M HCl for 30 seconds. After acidification, the sample was rinsed with 0.7 μm filtered seawater and dried by applying vacuum pressure to the filter. Each filter was transferred to its own aluminum foil envelope and stored at -20oC. Zooplankton samples were collected with a Bongo net (50 cm mouth diameter and 236 µm mesh) hauled vertically from 250 m depth. The zooplankton sample collected at each station was size fractionated using a sieve stack with 4 mm, 2 mm, 1 mm, 0.5 mm and 0.25 mm mesh sizes, and then frozen at -40°C. Samples of salmon, squids, jellyfish, myctophids and other fishes were obtained using a surface trawl (~120 m2, 30m deep x 40m wide) deployed at each station and towed at 4-5 knots for one hour. All organisms caught in the trawl were identified to the lowest practical taxon (species except for some invertebrates), enumerated, and measured (length [total, fork, mantle, or bell diameter as appropriate] to the nearest 1 mm, weight to the nearest 1 g). For salmonids, a 2x2 cm piece of muscle tissue was collected from above the lateral line and in front of the dorsal fin and stored at -40°C. For large non-salmonid fish, a muscle sample was collected in the same way as for salmonids samples. A 2x2 cm piece of muscle from the anterior dorsal margin of the mantle was collected from squids, and jellyfish were collected either whole or a piece in the case of large specimens. For micronekton species, either a piece of muscle was sampled (e.g., myctophids posterior region; squids’ mantle) or the specimen was analysed whole (e.g., krill, small jellyfish). All samples were stored frozen at -20○C. Samples were then processed in the laboratory at the University of British Columbia. POM Filters were oven dried at 50°C and later encapsulated for isotopic analysis. POM ẟ13C values are reported from the set of acidified samples, while ẟ15N are reported from non-acidified samples. Animal samples were either oven dried at 50°C (zooplankton, squids, myctophids and non-salmonid fishes) or freeze-dried (salmon and jellyfish), and then homogenized to a fine powder using mortar and pestle. Approximately 1 mg of each sample was encapsulated in tin caps and sent for carbon and nitrogen analysis at the UC Davis stable isotope facility (Davis, CA, USA). Tissue samples were analysed using an elemental analyser (PDZ Europa ANCA-GSL) interfaced to an isotope ratio mass spectrometer (PDZ Europa 20-20, Sercon Ltd., Cheshire, UK). Details on analytical procedure are provided in the supplementary materials 1. The data reported includes each sample ẟ13C (raw and lipid corrected, see supplementary material 1 and 2), total C content (mg), ẟ15N, total N content (mg), CN ratios, calculated trophic positions (see supplementary material 3), and salmon condition factor (supplementary material 4), in addition to the metadata associated with each sample (e.g., coordinates of oceanographic station and bottom depth where specimens were collected, etc.).

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Licence: Creative Commons Attribution 4.0

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Metadata Reference Date(s) February 20, 2024 (Publication)
February 20, 2024 (Revision)
Data Reference Date(s) February 21, 2019 (Creation)
Frequency of Update As Needed

Responsible Party 1
Affiliation
North Pacific Anadromous Fish Commission ROR logo
Email
secretariat@npafc.org
Role
Publisher
Responsible Party 2
Name
Troina, Genyffer Cibele ORCID logo
Affiliation
University of British Columbia ROR logo
Email
g.troina@oceans.ubc.ca
Role
  • Author
  • Custodian
  • Owner
  • Point of Contact
Responsible Party 3
Name
Foster, Mira
Affiliation
University of British Columbia ROR logo
Email
mirafoster98@gmail.com
Role
Co Author
Responsible Party 4
Name
Graham, Nicole
Affiliation
University of British Columbia ROR logo
Email
nicole.reid.graham@gmail.com
Role
Co Author
Responsible Party 5
Name
Link, Nicole
Affiliation
University of British Columbia ROR logo
Email
nlink@chem.ubc.ca
Role
Co Author
Responsible Party 6
Name
Pakhomov, Evgeny
Affiliation
University of British Columbia ROR logo
Email
epakhomov@eoas.ubc.ca
Role
Co Author
Responsible Party 7
Name
Portner, Lauren
Affiliation
University of British Columbia ROR logo
Email
l.portner@oceans.ubc.ca
Role
Co Author
Responsible Party 8
Name
Hunt, Brian ORCID logo
Affiliation
University of British Columbia ROR logo
Email
b.hunt@oceans.ubc.ca
Role
  • Owner
  • Principal Investigator
  • Co Author
Responsible Party 9
Affiliation
Hakai Institute ROR logo
Email
data@hakai.org
Role
Distributor

Field Value
Ocean Variables Stable carbon isotopes
Scope Dataset
Status Completed
Maintenance Note Generated from https://cioos-siooc.github.io/metadata-entry-form
Spatial Extent [[[-128.513667, 48.311], [-143.000167, 56.668667], [-147.503333, 56.667333], [-147.500667, 47.668333], [-136.997, 47.666833], [-129.9175, 48.191167], [-128.513667, 48.311]]]
North Bounding Latitude 47.666833
South Bounding Latitude 56.668667
East Bounding Longitude -128.513667
West Bounding Longitude -147.503333
Temporal Extent
Begin
2019-02-21
End
2019-03-14
Vertical Extent
Min
0.0
Max
250.0
Default Locale English
Included in Data Catalogue
Included in Data Catalogue 1
Name
International Year of the Salmon
Description
 
URL
https://data.npafc.org